Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. 3. Path Check Pathlength Measurement We would like to show you a description here but the site won’t allow us. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. The corrected absorbance is within 5% of the value. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. reader (FI-FLRH) SpectraMax Paradigm. 8 x 10-15. The dual monochromator optics allow. 00. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. For SpectraMax L or LMax II, LMax II 384 - no adapter used. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. October 2023. Meso Scale Discovery 1250 Sector Imager 2400 $ 2,500. -- JULY 19, 2016 Cables. • SpectraMax M5e Multi‐Mode Microplate Reader • FlexStation® 3 Multi‐Mode Microplate Reader Overview. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. , contact Molecular. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. 5. 8. 9000-0756. The following day, calcium content was quantified by the QuantiChrom™ Calcium Kit (BioAssay Systems) by reding absorbance at 612 nm using the Spectramax M5e reader (Molecular Devices). Molecular. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices. Molecular Devices SpectraMax M5e Multimode Microplate Reader. 97 cAMP Detection AssayThe SpectraMax M5e reader offers the additional benefit of being certified . You must scan the fluorochrome of interest in the SpectraMax M5 or M5e reader to determine the optimal excitation and emission wavelengths for your application. It was difficult and time consuming to undertake quality, repetitive research. Note: In this user guide, all references to SpectraMax Multi-Mode. Path Check Pathlength Measurement Multimode Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Multimode Readers Comparison Chart. The SpectraMax M2 and M2e are versatile and reliable. 1. Proceed with the following steps using optimized settings. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. FlexStation II 384. Find regional support. WARNING! The instrument weighs approximately 36 pounds. No. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax Paradigm reader. For more information please visit us at: SpectraMax®i3Multi-ModeMicroplateReader* SpectraMax®i3xMulti-ModeMicroplateReader*. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 00We would like to show you a description here but the site won’t allow us. Plate chamber design of the SpectraMax category 1 microplate readers. Protein melting curves were recorded using CFX96 thermocycler (Bio-Rad). 1. SpectraMax Mini: 17. Features. SpectraMax® Aquamax. Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and other niche laboratory equipment lines. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. In the M2, M5 and Plus 384, the light. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Devices SpectraMax M5e (Ex/Em 380/650nm) • SoftMaxPro software allows easy data analysis Real-time data analysis SoftMaxPro software allows easy viewing of oxygen consumption profiles in all wells (Fig. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. Categories:. SpectraMax M5e: 118. SpectraMax M5/M5e/M4/M3/M2/M2e. 2 x 10-14. D. book Page 55 Wednesday, July 7, 2010 2:09 PM. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. This document is provided to customers who have purchased Molecular Devices, Inc. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). book Page 1 Wednesday, July 7, 2010 2:09 PM The Molecular Devices SpectraMax M5e Microplate Reader enables five modes of detection. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. 5. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFor calcium content quantification, separate plates were used. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. The optical systemsThe BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. com A. 5. Benchmark Scientific Accuris SmartReader 96 MR9600 *NEW* Microplate Absorbance Reader. Description 1. 1. Paradigm: W = 21. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectramax i3x multi-mode detection platformMolecular devices spectramax m5e manual I3x spectramax microplate reader multi mode molecular devices minimax cytometer imaging selectscienceSpectramax i3 injury biomechanics medicine materials center. Delivers single mode reader performance in one multimode reader package. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. A neurosurgeon from Mercer Island and a noted mountaineer from Spokane, Washington, were killed on February 4, 1986, when. Your lab might even have one already. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Microplate Reader Shelf. 5 mM IPTG using a SpectraMax M5E. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. Configuring the SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers for ValitaTiter Assays. 8 x 10-15. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 1. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Moving a SpectraMax Multi-Mode Microplate Reader. When a feature or capability applies to only certain readers, this exception is noted. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Meso Scale Discovery 1200 Sector Imager 2400. 多功能酶标仪 FlexStation3 快速动力学操. 1. the SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. com. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. This Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader is in good working order. 3. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. No disponible por el momento. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Upgradeable modules are also available including western blot, cell imaging, and fast. HTRF assay performance on the SpectraMax M5e reader is demonstrated here with two different HTRF assay kits, cAMP dynamic 2 and IP-One. Italic and boldface type are used for emphasis. 8 x 10-15. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. D 1 1. With optimized reagents, option to operate the readers. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available) ATG for AKTA Explorer, Purifier and FPLC PMs, Repairs and Service Contracts. e. AVALANCHE. igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. 1. We would like to show you a description here but the site won’t allow us. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Thermo Lab Systems Multiskan Ascent 354 $1,400. 3. This is a used Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. The optical systemsSpectraMax L: 36. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax Plus384 SpectraMax 340PC384 SpectraMax 190. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. NanoRacks reconfigured the SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. M5e = 174nM SpectraMax M5e delta F % Paradigm = 18nM M5e = 19nM Figure 1. 8 x 10-15. Both instruments demonstrated good performance for the assay. 312 gain calibration failed. 00 - Original price $5,513. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. 1. Page 75. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Results obtained with SpectraMax iD5 (red), i3x (green), and M5e (blue) readers were plotted using a 4-parameter logistic, and EC 50 values of 0. com A. The large analog PMT works with monochromators and is used during fluorescence. Labsystems Fluoroskan Ascent FL Microplate Fluorometer. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. book Page 21 Wednesday, July 7, 2010 2:09 PM. Other technologies such as imaging. 40266 Email: drugdiscoverytech@lifetech. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Molecular Devices SpectraMax® series microplate readers. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 6 M HCl for 24 h. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. 508/20. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. 8. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. SpectraMax Mini: 17. NanoRacks LLC officials have chosen Molecular Devices’ SpectraMax M5e Multi-Mode Microplate Reader for use in NASA's International Space Station. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Results summary for cAMP HiRange standard curve Figure 3. The corrected absorbance is within 5% of the. 40266 Email: [email protected]. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s. 00 USD. The dual monochromator optics allow. This assay may also be detected on the SpectraMax ® i3 and SpectraMax ® M5e Multi-Mode Microplate ReadersThe SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. When a feature or capability applies to only certain readers, this exception is noted. 1. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. Gemini EM. book Page 1 Tuesday,. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Dual monochromators for assay flexibility. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular Devices. Description 1. 91. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Microplate Readers include the M3, M4, exception is noted. for Cisbio Bioassays HTRF® technology. SPECTRAMAX XS XENON REPLACEMENT BULB FOR MOLECULAR DEVICES SPECTRAMAX XPS XENON, SPECTRAMAX XS. View the Molecular Devices website. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart Features We would like to show you a description here but the site won’t allow us. spr SpectraMax Plus 384. 0200-7027. 5 Z’ factor 0. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Optimal instrument settings are the same for all three readers. The SpectraMax iD5 and SpectraMax i3x readers use SoftMax® Pro Data Acquisition and Analysis Software. StakMax Handling Stacker Base Unit. System setup and calibration are controlled. 40266 Email: drugdiscoverytech@lifetech. 1) SpectraMax Glo Steady Luc Reporter Assay (6. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. 8 x 10-15. SpectraMax Gemini EM/XPS. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Molecular Devices SpectraMax M5e Microplate Reader. Jun 9th, 2023. 72 MB. Setup Guide On The Molecular Devices SpectraMax®SpectraMax L: 36. Explore PDF for more details. HEK293 cells in growth medium (MEM/10% FBS/1% penicillin/streptomycin) were seeded at 50,000 cells per well, 50 μL per well, in a 96-well poly-L-lysine-coated microplate. 1(a). With the cAMP dynamic 2 kit, cAMP levels are detected using a Eu. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. . The optical systemsApplication note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Lector de microplacas M5E-SC SpectraMax M5e con contrato de servicio de 1 año - Lector de placas multimodo SpectraMax M5/M5e, dispositivos moleculares - cada uno . 00 USD. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. For wavelengths in the UV range above 220 nm,. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. Supplier: Molecular Devices. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. 1. 2 x 10-14. 3. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. The Gemini XS can read a variety of sample formats from 6- to 384-well. Introduction. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. book Page 1 Wednesday, July 7, 2010 2:09 PM Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax L: 36. 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. The optical systemsThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. . Optimal instrument settings are the same for all three readers. 1. 1, left panel) and individual wells (Fig. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 4. 1. Wide. The plate was agitated briefly, incubated 15 minutes at room temperature in the dark and read on M5e. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Listing SKU: DIS-58825-molecular-devices-spectramax-m5e-microplate-readerIn May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader in space. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. Introduction. SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes Generation of NADPH was monitored by fluorescence (Ex340/Em460 nm) every minute for 15 minutes on a SpectraMax M5e plate reader (Molecular Devices at 25°C). 1. book Page 1 Wednesday, July 7, 2010 2:09 PM. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. The mirror set. for Cisbio Bioassays HTRF® technology. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. This document is provided to customers who have purchased Molecular Devices, Inc. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. Following the user manual for installation, maintenance, assembly or troubleshoot is an crucial prerequisite for safety in addition to protect consumers from any liability from the manufacturer of the product for viable defects in case of an insurance claim. UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range. The following document is intended. 3. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). M5e: W = 15. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMulti-mode Microplate Readers. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Upgradeable modules are also available including western blot, cell imaging, and fast. Molecular Devices SpectraMax M5e User Manual (20 pages) Multi-Mode Microplate Readers. $. book Page 1 Wednesday, July 7, 2010 2:09 PM. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. Example: [Drawer]. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. 8 x 10-15. ZERO BIAS - scores, article reviews, protocol conditions and more-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Bioz Stars score: 86/100, based on 1 PubMed citations. for Cisbio Bioassays HTRF® technology. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Homogeneous Time-Resolved Fluorescence (HTRF) . Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 1. 0001 0. Choose cables that meet the requirement described in this appendix. Qualify the fluorescence performance of the SpectraMax . measured using a microplate reader (SpectraMax M5e, Molecular Devices, San Jose, CA, USA) with excitation and emission at 494 and 520 nm, respectively, and calibrated against a standard curve for FITC. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s. For more information about the SpectraMax® M5e Multi-Mode Microplate Reader or other products from Molecular Devices, please visit our website (or send an email to. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. -- JULY 19, 2016Cables. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 8 x 10-15. These were dispensed directly into a microplate and did not require any thermal cycling. spr SpectraMax iD3 SpectraTest ABS2 v1. SpectraMax Paradigm. The Spectr. In this application note we show how the SpectraMax ® i3, SpectraMax ® Paradigm ®, and SpectraMax ® M5e Multi-Mode Microplate Readers are used to perform robust, high-throughput HTRF ® assays with excellent Z’ factors and highly reproducible EC 50 values. D 47 7. Download PDF. 1. elegans, and Alcanivorax borkumensis. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well7. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. , 19 May 2011. The guide also covers the software features, the specifications, and the accessories of the systems. M3-M5e_manual. 3. For more sample throughput, the system canSetup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 7°C and the bottom to 36. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical-based and cell-Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader Chosen to be the First Microplate Reader in Space by NanoRacks, LLC for NASA’s International Space Station Sunnyvale, CA – May 17th, 2011 – Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research,Configure your microplate reader. , May 17, 2011 /PRNewswire/ -- Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the. Supplier: Molecular Devices. . Rating: 5. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. View full details Original price $5,513. SpectraMax M3, M4, M5, and M5 e. Lift up the lid over the chamber to insert or remove a cuvette. The M5 has two PMTs. Endpoint, kinetic, spectrum. 1(a). 5, Z’ = 0. Multi-Mode Microplate Readers. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. AquaMax® 2000. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Paradigm: W = 21. 8. It does not work as expected. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Add to Cart. SpectraMax M5e: 118. 97 cAMP Detection AssayMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader with One Year Warranty. Read mode. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The absorbance of the wells containing only the primary antibodies but no antigen was used as the background value. 8. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The validation kit included individual tubes of diluted fluorophores: FAM, HEX, and FAM+HEX. The plate reader. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Please see the exact video and photos below. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading.